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[heading]Research services[/heading]
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Protein Expression & Purification

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In silico drug design

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X-ray crystal structure

Protein Expression & Purification

개발공정 및 서비스범위

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발현용 벡터 cloning 서비스

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  • 소요기간: ~2주
  • 기본 제공 벡터
    • pET21a
    • pET24a
    • pET32a
  • Agarose gel 및 sequencing 결과 제공

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발현 평가

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  • 소요기간: ~1주
  • Expression system
    • E. coli
    • Mammalian cell (CHO)
  • SDS-PAGE 결과 제공

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배양

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  • 소요기간: ~1주
  • Scale: 0.5-20 L

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정제

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  • 소요기간: ~1주
  • Affinity
  • IEX
  • HIC
  • SEC
  • 순도 >90-98%
  • SDS-PAGE, Analytical SEC
    결과 제공

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In silico drug design

Protein drug design

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  • Mediate protein-protein interaction
  • Enhancing protein stability
  • Peptide mimic compound design

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Small-molecule drug design

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  • Protein-ligand interaction prediction
  • Ligand binding site prediction
  • Lead optimization

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X-ray crystal structure

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  • To obtain a three-dimensional structure of proteins
  • To analysis function of proteins or protein-ligand interactions
  • To use modifying drugs for enhancing potency or specificity

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Crystallization

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  • Initial crystal condition screening
  • Optimize conditions for crystal growth
  • Co-crystallization or soaking of ligands

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X-ray crystallography

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  • X-ray diffraction
  • Scaled & merged diffraction data
  • Phasing or molecular replacement
  • Model refinement

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